Biocontrol Activity, Mode of Action, and Colonization of Aureobasidium pullulans Dmg 30 DEP on Controlling Early Blight Disease on Tomato Plant

We investigated the biocontrol activity of A. pullulans Dmg 30 DEP against Alternaria solani causal agent early blight. Biocontrol was tested by in vivo and ad planta. were investigating antibiosis capabilities with dual culture method, paper dish assay, two-compartment petri trapping identification volatile organic compounds (VOCs) GC-MS. Lysis examined observing clear zone formed growing yeast on chitin agar skim milk agar. The ability hyperparasitism assessed block observed light microscopy scanning electron (SEM). results showed that plays a role suppression blight disease at 106 cells/ml 107 cell density. mechanism involved is production VOCs, chitinase protease enzymes, siderophore hyperparasitism. result shows colonizing tomato leaves following areole.